ARG82326

Human CCL4 / MIP1 beta ELISA Kit

Human CCL4 / MIP1 beta ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82326-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82326-002 Standard 2 X 10 ng/vial 4°C
ARG82326-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82326-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82326-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82326-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82326-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82326-008 25X Wash buffer 20 ml 4°C
ARG82326-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82326-010 STOP solution 10 ml (Ready to use) 4°C
ARG82326-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82326 Human CCL4 / MIP1 beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CCL4 / MIP1 beta in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CCL4 / MIP1 beta
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 3.9 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 7.8 - 500 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%
Inter-Assay CV: 6.2%
Alternate Names ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6351 Human CCL4

Swiss-port # P13236 Human C-C motif chemokine 4

Gene Symbol CCL4
Gene Full Name C-C motif chemokine ligand 4
Background The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]
Function Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. [UniProt]
Cellular Localization Secreted. [UniProt]
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Title Download Link
ARG82326 Human CCL4 / MIP1 beta ELISA Kit User manual download icon Download