ARG81762

Human CD10 ELISA Kit

Human CD10 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81762-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81762-002 Standard 2 X 10 ng/vial 4°C
ARG81762-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81762-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81762-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81762-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81762-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81762-008 25X Wash buffer 20 ml 4°C
ARG81762-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81762-010 STOP solution 10 ml (Ready to use) 4°C
ARG81762-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81762 Human CD10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD10 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD10
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 62.5 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.1%; Inter-Assay CV: 7.4%
Alternate Names Enkephalinase; Neutral endopeptidase; Neutral endopeptidase 24.11; Common acute lymphocytic leukemia antigen; Neprilysin; CD antigen CD10; CALLA; EC 3.4.24.11; CD10; Skin fibroblast elastase; SFE; Atriopeptidase; NEP

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4311 Human MME

Swiss-port # P08473 Human Neprilysin

Gene Symbol MME
Gene Full Name membrane metallo-endopeptidase
Background CD10 is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017]
Function CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573). [UniProt]
Highlight Related products:
CD10 antibodies; CD10 ELISA Kits; CD10 Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Myristoylation is a determinant of membrane targeting.

Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity. [UniProt]
Title Download Link
ARG81762 Human CD10 ELISA Kit User's manual download icon Download