ARG81694

Human CD105 / Endoglin ELISA Kit

Human CD105 / Endoglin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81694-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81694-002 Standard 2 X 10 ng/vial 4°C
ARG81694-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81694-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81694-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81694-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81694-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81694-008 25X Wash buffer 20 ml 4°C
ARG81694-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81694-010 STOP solution 10 ml (Ready to use) 4°C
ARG81694-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81694 Human CD105 / Endoglin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD105 / Endoglin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD105 / Endoglin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.7%; Inter-Assay CV: 6.3%
Alternate Names CD antigen CD105; HHT1; Endoglin; ORW1; END

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2022 Human ENG

Swiss-port # P17813 Human Endoglin

Gene Symbol ENG
Gene Full Name endoglin
Background This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
Function Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. [UniProt]
Highlight Related products:
CD105 antibodies; CD105 ELISA Kits;
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Title Download Link
ARG81694 Human CD105 / Endoglin ELISA Kit User's manual download icon Download