ARG83314
Human CD107b / LAMP2 ELISA Kit
Human CD107b / LAMP2 ELISA Kit for ELISA and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | ARG83314 Human CD107b / LAMP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD107b / LAMP2 in Serum, Plasma and Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD107b / LAMP2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 150 pg/ml |
| Detection Range | 312 pg/ml - 20,000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 3.6% Inter-Assay CV: 5.9% |
| Alternate Names | CD antigen CD107b; LAMPB; CD107b; LGP110; Lysosome-associated membrane glycoprotein 2; LAMP-2; CD107 antigen-like family member B; Lysosome-associated membrane protein 2 |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13473 Human Lysosome-associated membrane glycoprotein 2 |
|---|---|
| Gene Symbol | LAMP2 |
| Gene Full Name | lysosomal-associated membrane protein 2 |
| Background | CD107b (lysosome-associated membrane protein-2, LAMP-2), together with CD107a / LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, especially CD107b is important regulator in successful phagosomal maturation. CD107b deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danons disease). Immature CD107b is an approximately 45 kDa protein, but after extensive glycosylation the mature glycoprotein has about 100-120 kDa. |
| Function | Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| PTM | O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. |
| Title | Download Link |
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| ARG83314 Human CD107b / LAMP2 ELISA Kit User manual |
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