ARG70252

Human CD123 / IL3RA recombinant protein (ECD) (His-tagged, C-ter)

Human CD123 / IL3RA recombinant protein (ECD) (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Human CD123 / IL3RA recombinant protein (ECD).
Tested Reactivity Hu
Tested Application Binding, SDS-PAGE
Target Name CD123 / IL3RA (ECD)
Species Human
A.A. Sequence Lys20 - Arg305 of Human CD123 / IL3RA (NP_002174.1) with 6X His tag at the C-terminus.
Expression System HEK293
Alternate Names IL-3R subunit alpha; CD123; CD antigen CD123; IL3RAY; IL-3R-alpha; IL3RY; IL3RX; IL3R; IL-3RA; IL-3 receptor subunit alpha; hIL-3Ra; Interleukin-3 receptor subunit alpha

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IL3 at 4µg/ml (100µl/well) can bind recombinant Human IL3RA, the EC50 of Human IL3RA is 5.3 µg/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 90% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3563 Human IL3RA

Swiss-port # P26951 Human Interleukin-3 receptor subunit alpha

Gene Symbol IL3RA
Gene Full Name interleukin 3 receptor, alpha (low affinity)
Background The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]
Function This is a receptor for interleukin-3. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 43 kDa