ARG81316

Human CD130 ELISA Kit

Human CD130 ELISA Kit for ELISA and Human

Overview

Product Description ARG81316 Human CD130 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD130 in serum, plasma and cell culture supernatant.
Tested Reactivity Hu
Tested Application ELISA
Specificity Recognizes both natural and recombinant Human soluble CD130. No cross reactivity with other Human soluble receptors. No interference with neither soluble gp80 nor IL-6.
Target Name CD130 / gp130
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 56.25 - 1800 pg/ml
Sample Volume 100 µl
Alternate Names CDw130; CD130; CDW130; Interleukin-6 signal transducer; CD antigen CD130; IL-6RB; Membrane glycoprotein 130; GP130; Oncostatin-M receptor subunit alpha; IL-6R subunit beta; Interleukin-6 receptor subunit beta; gp130; IL-6 receptor subunit beta; IL-6R-beta

Application Instructions

Assay Time ~ 2 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3572 Human IL6ST

Swiss-port # P40189 Human Interleukin-6 receptor subunit beta

Gene Symbol IL6ST
Gene Full Name interleukin 6 signal transducer
Background The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]
Function Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. [UniProt]
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PTM Phosphorylation of Ser-782 down-regulates cell surface expression.

Heavily N-glycosylated (PubMed:11098061, PubMed:16335952, PubMed:19159218, PubMed:19139490, PubMed:11251120). Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding (PubMed:19915009). [UniProt]
Title Download Link
ARG81316 Human CD130 ELISA Kit User's manual download icon Download