ARG70103
Human CD137L / TNFSF9 recombinant protein (Active) (His-tagged, C-ter)
Human CD137L / TNFSF9 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human CD137L / TNFSF9 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CD137L / TNFSF9 |
| Species | Human |
| A.A. Sequence | MREGP ELSPD DPAGL LDLRQ GMFAQ LVAQN VLLID GPLSW YSDPG LAGVS LTGGL SYKED TKELV VAKAG VYYVF FQLEL RRVVA GEGSG SVSLA LHLQP LRSAA GAAAL ALTVD LPPAS SEARN SAFGF QGRLL HLSAG QRLGV HLHTE ARARH AWQLT QGATV LGLFR VTPEI PAGLP SPRSE with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 0.7 ng/ml. |
| Alternate Names | 4-1BBL; 4-1BB ligand; Tumor necrosis factor ligand superfamily member 9; 4-1BB-L; CD137L |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TNFSF9 |
|---|---|
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 9 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.[provided by RefSeq, Oct 2008] |
| Function | Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. [UniProt] |
| Cellular Localization | Membrane; Single-pass type II membrane protein. [UniProt] |
