ARG81317

Human CD14 ELISA Kit

Human CD14 ELISA Kit for ELISA and Human

Overview

Product Description

ARG81317 Human CD14 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD14 in serum, plasma and cell culture supernatant.

Tested Reactivity Hu
Tested Application ELISA
Specificity Recognizes both natural and recombinant Human soluble CD14. No cross reactivity with other Human soluble molecules.
Target Name CD14
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 6 ng/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 6.875 - 220 ng/ml
Sample Volume 100 µl
Alternate Names CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14

Application Instructions

Assay Time ~ 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 929 Human CD14

Swiss-port # P08571 Human Monocyte differentiation antigen CD14

Gene Symbol CD14
Gene Full Name CD14 molecule
Background The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]
Highlight Related products:
CD14 antibodies; CD14 ELISA Kits; CD14 Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. [UniProt]
Title Download Link
ARG81317 Human CD14 ELISA Kit User's manual download icon Download