ARG81996

Human CD146 ELISA Kit

Human CD146 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81996-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81996-002 Standard 2 X 10 ng/vial 4°C
ARG81996-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81996-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81996-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81996-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81996-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81996-008 25X Wash buffer 20 ml 4°C
ARG81996-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81996-010 STOP solution 10 ml (Ready to use) 4°C
ARG81996-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81996 Human CD146 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD146 in serum and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD146
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.9%; Inter-Assay CV: 6.2%
Alternate Names Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; Cell surface glycoprotein MUC18; Melanoma cell adhesion molecule; CD146; MUC18; CD antigen CD146; S-endo 1 endothelial-associated antigen

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4162 Human MCAM

Swiss-port # P43121 Human Cell surface glycoprotein MUC18

Gene Symbol MCAM
Gene Full Name melanoma cell adhesion molecule
Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. [UniProt]
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Title Download Link
ARG81996 Human CD146 ELISA Kit User's manual download icon Download