ARG82605

Human CD152 / CTLA4 ELISA Kit

Human CD152 / CTLA4 ELISA Kit for ELISA and Human

Overview

Product Description ARG82605 Human CD152 / CTLA4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD152 / CTLA4 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity This kit could assay both natural and recombinant Human CD152 / CTLA4.

No significant cross-reactivity or interference was observed in the following samples:
Human: IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, TGF beta 1, TNF alpha and VEGF.
Mouse: GM-CSF, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha.
Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha.
Target Name CD152 / CTLA4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 46.9 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 93.8 - 6000 pg/ml
Sample Volume 50 µl
Precision Intra-Assay CV: 3.1%
Inter-Assay CV: 3.7%
Alternate Names GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12

Application Instructions

Assay Time ~ 3.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1493 Human CTLA4

Swiss-port # P16410 Human Cytotoxic T-lymphocyte protein 4

Gene Symbol CTLA4
Gene Full Name cytotoxic T-lymphocyte-associated protein 4
Background This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;. [UniProt]
Highlight Related products:
CD152 antibodies; CD152 ELISA Kits;
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PTM N-glycosylation is important for dimerization.

Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. [UniProt]
Title Download Link
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