ARG82512
Human CD155 / Poliovirus Receptor ELISA Kit
Human CD155 / Poliovirus Receptor ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82512-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82512-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82512-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82512-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82512-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82512-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82512-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82512-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82512-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82512-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82512-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82512 Human CD155 / Poliovirus Receptor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD155 / Poliovirus Receptor in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CD155 / Poliovirus Receptor |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.8% Inter-Assay CV: 6.3% |
| Alternate Names | NECL5; CD antigen CD155; Nectin-like protein 5; PVS; Necl-5; HVED; Poliovirus receptor; TAGE4; NECL-5; CD155 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PVR |
| Gene Full Name | poliovirus receptor |
| Background | The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
| Function | Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. [UniProt] |
| Cellular Localization | Isoform Alpha: Cell membrane; Single-pass type I membrane protein. Isoform Delta: Cell membrane; Single-pass type I membrane protein. Isoform Beta: Secreted. Isoform Gamma: Secreted. [UniProt] |
| Highlight | Related products: CD155 antibodies; CD155 ELISA Kits; CD155 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4. Phosphorylated by Src kinases on tyrosine residues in the ITIM motif upon ligation. Interaction with TIGIT is required for Phosphorylation. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82512 Human CD155 / Poliovirus Receptor ELISA Kit User manual |
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