ARG82389

Human CD164 ELISA Kit

Human CD164 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82389-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82389-002 Standard 2 X 10 ng/vial 4°C
ARG82389-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82389-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82389-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82389-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82389-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82389-008 25X Wash buffer 20 ml 4°C
ARG82389-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82389-010 STOP solution 10 ml (Ready to use) 4°C
ARG82389-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82389 Human CD164 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD164 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CD164
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 50 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 93.8 - 6000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 6.7%
Alternate Names MGC-24; Multi-glycosylated core protein 24; CD antigen CD164; Endolyn; endolyn; Sialomucin core protein 24; MGC-24v; MUC-24

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8763 Human CD164

Swiss-port # Q04900 Human Sialomucin core protein 24

Gene Symbol CD164
Gene Full Name CD164 molecule, sialomucin
Background Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play 2 key but opposing roles in vivo: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor (Watt et al., 1998 [PubMed 9680353]; Forde et al., 2007 [PubMed 17077324]).[supplied by OMIM, Aug 2008]
Function Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34(+) cells to the stroma and by negatively regulating CD34(+)CD38(lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediated through the CXCL12/CXCR4 axis. May play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells. Promotes myogenesis by enhancing CXCR4-dependent cell motility. Positively regulates myoblast migration and promotes myoblast fusion into myotubes (By similarity). [UniProt]
Cellular Localization Lysosome membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt]
Highlight Related products:
CD164 antibodies; CD164 ELISA Kits;
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PTM Highly N- and O-glycosylated; contains sialic acid.

The motif Ser-Gly may serve as the site of attachment of a glycosaminoglycan side chain. [UniProt]
Title Download Link
ARG82389 Human CD164 ELISA Kit User manual download icon Download