ARG81972

Human CD170 / Siglec 5 ELISA Kit

Human CD170 / Siglec 5 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81972-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81972-002 Standard 2 X 10 ng/vial 4°C
ARG81972-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81972-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81972-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81972-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81972-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81972-008 25X Wash buffer 20 ml 4°C
ARG81972-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81972-010 STOP solution 10 ml (Ready to use) 4°C
ARG81972-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81972 Human CD170 / Siglec 5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD170 / Siglec 5 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD170 / Siglec 5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 23.45 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 46.9 - 3000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 6.4%
Alternate Names OBBP2; OB-binding protein 2; CD170; Sialic acid-binding Ig-like lectin 5; SIGLEC-5; CD antigen CD170; CD33 antigen-like 2; OB-BP2; CD33L2; Siglec-5; Obesity-binding protein 2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8778 Human SIGLEC5

Swiss-port # O15389 Human Sialic acid-binding Ig-like lectin 5

Gene Symbol SIGLEC5
Gene Full Name sialic acid binding Ig-like lectin 5
Background This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. [UniProt]
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Title Download Link
ARG81972 Human CD170 / Siglec 5 ELISA Kit User's manual download icon Download