ARG82515

Human CD200R ELISA Kit

Human CD200R ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82515-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82515-002 Standard 2 X 10 ng/vial 4°C
ARG82515-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82515-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82515-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82515-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82515-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82515-008 25X Wash buffer 20 ml 4°C
ARG82515-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82515-010 STOP solution 10 ml (Ready to use) 4°C
ARG82515-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82515 Human CD200R ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD200R in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CD200R
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 10 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.5%
Inter-Assay CV: 6.3%
Alternate Names MOX2R; Cell surface glycoprotein CD200 receptor 1; CD200R; Cell surface glycoprotein OX2 receptor 1; OX2R; HCRTR2; CD200 cell surface glycoprotein receptor

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 131450 Human CD200R1

Swiss-port # Q8TD46 Human Cell surface glycoprotein CD200 receptor 1

Gene Symbol CD200R1
Gene Full Name CD200 receptor 1
Background This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Function Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 4: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted. [UniProt]
Highlight Related products:
CD200R antibodies; CD200R ELISA Kits;
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Simplify the ELISA analysis by GainData
PTM The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform. [UniProt]
Title Download Link
ARG82515 Human CD200R ELISA Kit User manual download icon Download