ARG81889

Human CD222 ELISA Kit

Human CD222 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81889-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81889-002 Standard 2 X 10 ng/vial 4°C
ARG81889-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81889-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81889-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81889-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81889-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81889-008 25X Wash buffer 20 ml 4°C
ARG81889-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81889-010 STOP solution 10 ml (Ready to use) 4°C
ARG81889-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81889 Human CD222 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD222 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD222
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.7%; Inter-Assay CV: 6.2%
Alternate Names CD222; MPR 300; Insulin-like growth factor II receptor; M6P/IGF2R; MPRI; 300 kDa mannose 6-phosphate receptor; IGF-II receptor; CI Man-6-P receptor; M6P/IGF2 receptor; MPR1; CIMPR; Cation-independent mannose-6-phosphate receptor; CD antigen CD222; Insulin-like growth factor 2 receptor; M6P-R; CI-MPR; M6PR

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3482 Human IGF2R

Swiss-port # P11717 Human Cation-independent mannose-6-phosphate receptor

Gene Symbol IGF2R
Gene Full Name insulin-like growth factor 2 receptor
Background This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele. [provided by RefSeq, Apr 2013]
Function Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. [UniProt]
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CD222 antibodies; CD222 ELISA Kits;
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Title Download Link
ARG81889 Human CD222 ELISA Kit User manual download icon Download