ARG82332

Human CD23 ELISA Kit

Human CD23 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82332-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82332-002 Standard 2 X 10 ng/vial 4°C
ARG82332-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82332-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82332-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82332-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82332-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82332-008 25X Wash buffer 20 ml 4°C
ARG82332-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82332-010 STOP solution 10 ml (Ready to use) 4°C
ARG82332-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82332 Human CD23 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD23 in serum, plasma (EDTA, heparin), cell culture supernatants and urine.
Tested Reactivity Hu
Tested Application ELISA
Target Name CD23
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin), cell culture supernatants and urine.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 6.3%
Alternate Names CD23A; BLAST-2; Lymphocyte IgE receptor; CLEC4J; Immunoglobulin E-binding factor; CD23; IGEBF; Fc-epsilon-RII; CD antigen CD23; FCE2; Low affinity immunoglobulin epsilon Fc receptor; C-type lectin domain family 4 member J

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2208 Human FCER2

Swiss-port # P06734 Human Low affinity immunoglobulin epsilon Fc receptor

Gene Symbol FCER2
Gene Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Background The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Function Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). [UniProt]
Cellular Localization Cell membrane; Single-pass type II membrane protein. Cell membrane; Lipid-anchor. Secreted. Note=Also exists as a soluble excreted form, sCD23. [UniProt]
Highlight Related products:
CD23 antibodies; CD23 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N- and O-glycosylated.

The secreted form sCD23 is produced by ADAM10-mediated ectodomain shedding. [UniProt]
Title Download Link
ARG82332 Human CD23 ELISA Kit User manual download icon Download