ARG81779

Human CD244 ELISA Kit

Human CD244 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81779-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81779-002 Standard 2 X 10 ng/vial 4°C
ARG81779-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81779-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81779-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81779-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81779-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81779-008 25X Wash buffer 20 ml 4°C
ARG81779-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81779-010 STOP solution 10 ml (Ready to use) 4°C
ARG81779-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81779 Human CD244 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD244 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD244
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 10 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%; Inter-Assay CV: 7.8%
Alternate Names NK cell activation-inducing ligand; h2B4; Natural killer cell receptor 2B4; CD antigen CD244; 2B4; NAIL; NKR2B4; SLAM family member 4; Nmrk; SLAMF4; Signaling lymphocytic activation molecule 4; NK cell type I receptor protein 2B4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51744 Human CD244

Swiss-port # Q9BZW8 Human Natural killer cell receptor 2B4

Gene Symbol CD244
Gene Full Name CD244 molecule, natural killer cell receptor 2B4
Background This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Function Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family. [UniProt]
Highlight Related products:
CD244 antibodies; CD244 ELISA Kits;
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PTM N-linked glycosylation is essential for the binding to its ligand CD48. Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding.

Phosphorylated by FYN and CSK on tyrosine residues following activation. Coligation with inhibitory receptors such as KIR2DL1 inhibits phosphorylation upon contact of NK cells with sensitive target cells. [UniProt]
Title Download Link
ARG81779 Human CD244 ELISA Kit User's manual download icon Download