ARG81481

Human CD255 / TWEAK ELISA Kit

Human CD255 / TWEAK ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81481-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81481-002 Standard 2 X 10 ng/vial 4°C
ARG81481-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81481-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81481-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81481-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81481-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81481-008 25X Wash buffer 20 ml 4°C
ARG81481-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81481-010 STOP solution 10 ml (Ready to use) 4°C
ARG81481-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81481 Human CD255 / TWEAK ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD255 / TWEAK in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD255 / TWEAK
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%
Inter-Assay CV: 6.3%
Alternate Names APO3L; DR3LG; TWEAK; TNLG4A

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8742 Human TNFSF12

Swiss-port # O43508 Human Tumor necrosis factor ligand superfamily member 12

Gene Symbol TNFSF12
Gene Full Name TNF superfamily member 12
Background The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]
Function Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. [UniProt]
Cellular Localization Plasma membrane; Extracellular region or secreted. [UniProt]
Highlight Related products:
CD255 antibodies; CD255 ELISA Kits;
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Title Download Link
ARG81481 Human TWEAK ELISA Kit User's manual download icon Download