ARG70300
Human CD264 / TRAIL R4 recombinant protein (ECD) (Fc-His-tagged, C-ter)
Human CD264 / TRAIL R4 recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Human CD264 / TRAIL R4 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, SDS-PAGE |
| Target Name | CD264 / TRAIL R4 (ECD) |
| Species | Human |
| A.A. Sequence | Ala56 - His211 of Human CD264 / TRAIL R4 (NP_003831.2) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | Tumor necrosis factor receptor superfamily member 10D; CD264; DCR2; CD antigen CD264; DcR2; Decoy receptor 2; TNF-related apoptosis-inducing ligand receptor 4; TRUNDD; TRAILR4; TRAIL receptor with a truncated death domain; TRAIL receptor 4; TRAIL-R4 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human TRAIL at 2µg/ml (100 µl/well) can bind Recombinant Human DcR2 with a linear range of 15-60 ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UBN6 Human Tumor necrosis factor receptor superfamily member 10D |
|---|---|
| Gene Symbol | TNFRSF10D |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. [provided by RefSeq, Jul 2008] |
| Function | Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 42 kDa |
