ARG81713

Human CD27 ELISA Kit

Human CD27 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81713-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81713-002 Standard 2 X 10 ng/vial 4°C
ARG81713-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81713-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81713-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81713-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81713-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81713-008 25X Wash buffer 20 ml 4°C
ARG81713-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81713-010 STOP solution 10 ml (Ready to use) 4°C
ARG81713-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81713 Human CD27 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD27 in serum and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD27
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 7.3%
Alternate Names T-cell activation antigen CD27; Tp55; CD antigen CD27; CD27 antigen; S152. LPFS2; T14; S152; Tumor necrosis factor receptor superfamily member 7; CD27L receptor; TNFRSF7

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 939 Human CD27

Swiss-port # P26842 Human CD27 antigen

Gene Symbol CD27
Gene Full Name CD27 molecule
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
Function Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. [UniProt]
Highlight Related products:
CD27 antibodies; CD27 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Phosphorylated.

O-glycosylated with core 1 or possibly core 8 glycans. [UniProt]
Title Download Link
ARG81713 Human CD27 ELISA Kit User's manual download icon Download