ARG81792

Human CD271 ELISA Kit

Human CD271 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81792-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81792-002 Standard 2 X 10 ng/vial 4°C
ARG81792-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81792-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81792-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81792-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81792-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81792-008 25X Wash buffer 20 ml 4°C
ARG81792-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81792-010 STOP solution 10 ml (Ready to use) 4°C
ARG81792-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81792 Human CD271 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD271 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD271
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 5.9%
Alternate Names Gp80-LNGFR; CD271; CD antigen CD271; p75 ICD; Tumor necrosis factor receptor superfamily member 16; p75NTR; NGF receptor; TNFRSF16; p75(NTR); Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4804 Human NGFR

Swiss-port # P08138 Human Tumor necrosis factor receptor superfamily member 16

Gene Symbol NGFR
Gene Full Name nerve growth factor receptor
Background Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]
Function Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt]
Highlight Related products:
CD271 antibodies; CD271 ELISA Kits;
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PTM N- and O-glycosylated.

O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.

Phosphorylated on serine residues. [UniProt]
Title Download Link
ARG81792 Human CD271 ELISA Kit User's manual download icon Download