ARG70309

Human CD272 / BTLA recombinant protein (ECD) (Fc-His-tagged, C-ter)

Human CD272 / BTLA recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human

Overview

Product Description HEK293 expressed, Fc-His-tagged (C-ter) Human CD272 / BTLA recombinant protein (ECD).
Tested Reactivity Hu
Tested Application Binding, SDS-PAGE
Target Name CD272 / BTLA (ECD)
Species Human
A.A. Sequence Lys31 - Thr134 of Human CD272 / BTLA (NP_001078826.1) with an Fc-6X His tag at the C-terminus.
Expression System HEK293
Alternate Names CD antigen CD272; BTLA1; B- and T-lymphocyte-associated protein; B- and T-lymphocyte attenuator; CD272

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant human BTLA at 3µg/ml (100 µl/well) can bind Biotinylated Recombinant human HVEM with a linear range of 18-72 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 90% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 151888 Human BTLA

Swiss-port # Q7Z6A9 Human B- and T-lymphocyte attenuator

Gene Symbol BTLA
Gene Full Name B and T lymphocyte associated
Background This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]
Function Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044). [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 33 kDa
PTM Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.

N-glycosylated. [UniProt]