ARG70299
Human CD273 / PD-L2 recombinant protein (ECD) (Fc-His-tagged, C-ter)
Human CD273 / PD-L2 recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Human CD273 / PD-L2 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, SDS-PAGE |
| Target Name | CD273 / PD-L2 (ECD) |
| Species | Human |
| A.A. Sequence | Leu20 - Pro219 of Human CD273 / PD-L2 (NP_079515.2) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | B7DC; bA574F11.2; PDL2; CD antigen CD273; PD-L2; Programmed cell death 1 ligand 2; CD273; Btdc; PDCD1L2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; PD-1 ligand 2; PDCD1 ligand 2 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human PD-1 at 5µg/ml (100 µl/well) can bind Recombinant Human PD-L2 with a linear range of 0.06-0.24µg/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDCD1LG2 |
| Gene Full Name | programmed cell death 1 ligand 2 |
| Function | Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity). [UniProt] |
| Cellular Localization | Isoform 3: Secreted. Isoform 2: Endomembrane system; Single-pass type I membrane protein. Isoform 1: Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 31 kDa |
