ARG81502

Human CD278 / ICOS ELISA Kit

Human CD278 / ICOS ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81502-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81502-002 Standard 2 X 10 ng/vial 4°C
ARG81502-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81502-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81502-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81502-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81502-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81502-008 25X Wash buffer 20 ml 4°C
ARG81502-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81502-010 STOP solution 10 ml (Ready to use) 4°C
ARG81502-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81502 Human CD278 / ICOS ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD278 / ICOS in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD278 / ICOS
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%
Inter-Assay CV: 6.3%
Alternate Names CD antigen CD278; CD278; Inducible T-cell costimulator; AILIM; Activation-inducible lymphocyte immunomediatory molecule; CVID1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29851 Human ICOS

Swiss-port # Q9Y6W8 Human Inducible T-cell costimulator

Gene Symbol ICOS
Gene Full Name inducible T-cell co-stimulator
Background The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]
Function Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity). [UniProt]
Highlight Related products:
CD278 antibodies; CD278 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N-glycosylated. [UniProt]
Title Download Link
ARG81502 Human CD278 / ICOS ELISA Kit User's manual download icon Download