ARG81922

Human CD28 ELISA Kit

Human CD28 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81922-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81922-002 Standard 2 X 10 ng/vial 4°C
ARG81922-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81922-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81922-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81922-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81922-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81922-008 25X Wash buffer 20 ml 4°C
ARG81922-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81922-010 STOP solution 10 ml (Ready to use) 4°C
ARG81922-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81922 Human CD28 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD28 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD28
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 6.8%
Alternate Names Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 940 Human CD28

Swiss-port # P10747 Human T-cell-specific surface glycoprotein CD28

Gene Symbol CD28
Gene Full Name CD28 molecule
Background The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt]
Highlight Related products:
CD28 antibodies; CD28 ELISA Kits; CD28 Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM CD40LG induces tyrosine phosphorylation of isoform 3. [UniProt]
Title Download Link
ARG81922 Human CD28 ELISA Kit User's manual download icon Download