ARG81684

Human CD30 ELISA Kit

Human CD30 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81684-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81684-002 Standard 2 X 10 ng/vial 4°C
ARG81684-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81684-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81684-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81684-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81684-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81684-008 25X Wash buffer 20 ml 4°C
ARG81684-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81684-010 STOP solution 10 ml (Ready to use) 4°C
ARG81684-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81684 Human CD30 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD30 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD30
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 7.2%
Alternate Names Tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30; Ki-1; Lymphocyte activation antigen CD30; CD antigen CD30; D1S166E; CD30L receptor

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 943 Human TNFRSF8

Swiss-port # P28908 Human Tumor necrosis factor receptor superfamily member 8

Gene Symbol TNFRSF8
Gene Full Name tumor necrosis factor receptor superfamily, member 8
Background CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Function CD30 is a receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898). [UniProt]
Highlight Related products:
CD30 antibodies; CD30 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Phosphorylated on serine and tyrosine residues. [UniProt]
Title Download Link
ARG81684 Human CD30 ELISA Kit User's manual download icon Download