ARG82450

Human CD300a ELISA Kit

Human CD300a ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82450-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82450-002 Standard 2 X 10 ng/vial 4°C
ARG82450-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82450-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82450-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82450-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82450-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82450-008 25X Wash buffer 20 ml 4°C
ARG82450-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82450-010 STOP solution 10 ml (Ready to use) 4°C
ARG82450-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82450 Human CD300a ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD300a in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CD300a
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 5.9%
Alternate Names CMRF-35-H9; Inhibitory receptor protein 60; NK inhibitory receptor; CMRF35-H; CMRF35H9; CD antigen CD300a; IRC1/IRC2; IGSF12; CMRF35H; IRp60; IgSF12; CMRF35-like molecule 8; CMRF35-H9; CMRF-35H; CD300 antigen-like family member A; CLM-8; IRC1; IRC2; Immunoglobulin superfamily member 12

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11314 Human CD300A

Swiss-port # Q9UGN4 Human CMRF35-like molecule 8

Gene Symbol CD300A
Gene Full Name CD300a molecule
Background This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Function Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
Highlight Related products:
CD300 antibodies; CD300 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Phosphorylated on tyrosine.

N-glycosylated. [UniProt]
Title Download Link
ARG82450 Human CD300a ELISA kit User manual download icon Download