ARG70261
Human CD38 recombinant protein (ECD) (His-tagged, C-ter)
Human CD38 recombinant protein (ECD) (His-tagged, C-ter) for SDS-PAGE,ELISA,Binding Activity and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Human CD38 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, ELISA, SDS-PAGE |
| Target Name | CD38 (ECD) |
| Species | Human |
| A.A. Sequence | Val43 - Ile300 of Human CD38 (NP_001766.2) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | cADPr hydrolase 1; ADPRC 1; EC 3.2.2.6; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; Cyclic ADP-ribose hydrolase 1; ADPRC1; EC 2.4.99.20; ADP-ribosyl cyclase 1; 2'-phospho-cyclic-ADP-ribose transferase; CD antigen CD38; T10; 2'-phospho-ADP-ribosyl cyclase; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD38 at 0.5 ug/ml (100 µl/well) can bind CD38 antibody with a linear range of 2-10 ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28907 Human ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 |
|---|---|
| Gene Symbol | CD38 |
| Gene Full Name | CD38 molecule |
| Background | The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Function | Synthesizes the second messengers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. [UniProt] |
| Cellular Localization | Membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 34 kDa |
