ARG70333
Human CD40 recombinant protein (ECD) (Fc-His-tagged, C-ter)
Human CD40 recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Human CD40 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, SDS-PAGE |
| Target Name | CD40 (ECD) |
| Species | Human |
| A.A. Sequence | Glu21 - Arg193 of Human CD40 (NP_001241.1) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD40 Ligand at 2µg/ml (100 µl/well) can bind Recombinant Human CD40 with a linear range of 224-898 ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P25942 Human Tumor necrosis factor receptor superfamily member 5 |
|---|---|
| Gene Symbol | CD40 |
| Gene Full Name | CD40 molecule, TNF receptor superfamily member 5 |
| Background | This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014] |
| Function | Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity). [UniProt] |
| Cellular Localization | Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted. [UniProt] |
| Calculated MW | 31 kDa |
