ARG81919

Human CD47 ELISA Kit

Human CD47 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81919-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81919-002 Standard 2 X 10 ng/vial 4°C
ARG81919-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81919-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81919-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81919-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81919-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81919-008 25X Wash buffer 20 ml 4°C
ARG81919-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81919-010 STOP solution 10 ml (Ready to use) 4°C
ARG81919-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81919 Human CD47 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD47 in serum and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD47
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 62.5 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.9%; Inter-Assay CV: 6.4%
Alternate Names Leukocyte surface antigen CD47; CD antigen CD47; Antigenic surface determinant protein OA3; MER6; OA3; Protein MER6; IAP; Integrin-associated protein

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 961 Human CD47

Swiss-port # Q08722 Human Leukocyte surface antigen CD47

Gene Symbol CD47
Gene Full Name CD47 molecule
Background This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
Function Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. [UniProt]
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Title Download Link
ARG81919 Human CD47 ELISA Kit User's manual download icon Download