ARG81948

Human CD48 ELISA Kit

Human CD48 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81948-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81948-002 Standard 2 X 10 ng/vial 4°C
ARG81948-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81948-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81948-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81948-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81948-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81948-008 25X Wash buffer 20 ml 4°C
ARG81948-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81948-010 STOP solution 10 ml (Ready to use) 4°C
ARG81948-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81948 Human CD48 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD48 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD48
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.1%; Inter-Assay CV: 7.2%
Alternate Names Leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen; BCM1 surface antigen; CD antigen CD48; TCT.1; BLAST1; BCM1; MEM-102; mCD48; Signaling lymphocytic activation molecule 2; BLAST; hCD48; SLAMF2; SLAM family member 2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 962 Human CD48

Swiss-port # P09326 Human CD48 antigen

Gene Symbol CD48
Gene Full Name CD48 molecule
Background This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Function Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation. [UniProt]
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Title Download Link
ARG81948 Human CD48 ELISA Kit User's manual download icon Download