ARG70322

Human CD66e / CEA recombinant protein (ECD) (His-tagged, C-ter)

Human CD66e / CEA recombinant protein (ECD) (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Human CD66e / CEA recombinant protein (ECD).
Tested Reactivity Hu
Tested Application Binding, SDS-PAGE
Target Name CD66e / CEA (ECD)
Species Human
A.A. Sequence Lys35 - Ala685 of Human CD66e / CEA (NP_004354.2) with 6X His tag at the C-terminus.
Expression System HEK293
Alternate Names Carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; CD antigen CD66e; Carcinoembryonic antigen; Meconium antigen 100

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CEACAM5 at 5 µg/ml (100µl/well) can bind Recombinant Human CEACAM8 with a linear range of 0.5-3 µg/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1048 Human CEACAM5

Swiss-port # P06731 Human Carcinoembryonic antigen-related cell adhesion molecule 5

Gene Symbol CEACAM5
Gene Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Background This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Function Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).

(Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
Calculated MW 77 kDa
PTM Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. [UniProt]