ARG82742

Human CD69 ELISA Kit

Human CD69 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82742-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82742-002 Standard 2 X 10 ng/vial 4°C
ARG82742-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82742-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82742-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82742-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82742-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82742-008 25X Wash buffer 20 ml 4°C
ARG82742-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82742-010 STOP solution 10 ml (Ready to use) 4°C
ARG82742-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82742 Human CD69 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD69 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CD69
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 50 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.8%
Inter-Assay CV: 6.3%
Alternate Names GP32/28; Activation inducer molecule; MLR-3; BL-AC/P26; Leukocyte surface antigen Leu-23; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; EA1; CD antigen CD69; CLEC2C; C-type lectin domain family 2 member C

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 969 Human CD69

Swiss-port # Q07108 Human Early activation antigen CD69

Gene Symbol CD69
Gene Full Name CD69 molecule
Background This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]
Function Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. [UniProt]
Cellular Localization Membrane; Single-pass type II membrane protein. [UniProt]
Highlight Related products:
CD69 antibodies; CD69 ELISA Kits;
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Simplify the ELISA analysis by GainData
PTM Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. [UniProt]
Title Download Link
ARG82742 Human CD69 ELISA kit User manual download icon Download