ARG81920

Human CD73 ELISA Kit

Human CD73 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81920-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81920-002 Standard 2 X 10 ng/vial 4°C
ARG81920-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81920-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81920-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81920-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81920-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81920-008 25X Wash buffer 20 ml 4°C
ARG81920-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81920-010 STOP solution 10 ml (Ready to use) 4°C
ARG81920-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81920 Human CD73 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD73 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD73
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.0%; Inter-Assay CV: 7.0%
Alternate Names 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4907 Human NT5E

Swiss-port # P21589 Human 5'-nucleotidase

Gene Symbol NT5E
Gene Full Name 5'-nucleotidase, ecto (CD73)
Background The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt]
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Title Download Link
ARG81920 Human CD73 ELISA Kit User manual download icon Download