ARG81718

Human CD80 ELISA Kit

Human CD80 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81718-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81718-002 Standard 2 X 10 ng/vial 4°C
ARG81718-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81718-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81718-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81718-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81718-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81718-008 25X Wash buffer 20 ml 4°C
ARG81718-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81718-010 STOP solution 10 ml (Ready to use) 4°C
ARG81718-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81718 Human CD80 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD80 in serum and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD80
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%; Inter-Assay CV: 6.2%
Alternate Names B7.1; CTLA-4 counter-receptor B7.1; CD28LG; T-lymphocyte activation antigen CD80; B7-1; CD28LG1; B7; LAB7; Activation B7-1 antigen; CD antigen CD80; BB1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 941 Human CD80

Swiss-port # P33681 Human T-lymphocyte activation antigen CD80

Gene Symbol CD80
Gene Full Name CD80 molecule
Background The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
Function Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. [UniProt]
Highlight Related products:
CD80 antibodies; CD80 ELISA Kits;
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Title Download Link
ARG81718 Human CD80 ELISA Kit User's manual download icon Download