ARG82763

Human CD85e / LILRA3 ELISA Kit

Human CD85e / LILRA3 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82763-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82763-002 Standard 2 X 10 ng/vial 4°C
ARG82763-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82763-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82763-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82763-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82763-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82763-008 25X Wash buffer 20 ml 4°C
ARG82763-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82763-010 STOP solution 10 ml (Ready to use) 4°C
ARG82763-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82763 Human CD85e / LILRA3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD85e / LILRA3 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CD85e / LILRA3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.1%
Inter-Assay CV: 8.2%
Alternate Names ILT6; LIR-4; CD85 antigen-like family member E; ILT-6; Immunoglobulin-like transcript 6; LIR4; HM31; HM43; Monocyte inhibitory receptor HM43/HM31; CD85E; CD antigen CD85e; Leukocyte immunoglobulin-like receptor subfamily A member 3; Leukocyte immunoglobulin-like receptor 4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11026 Human LILRA3

Swiss-port # Q8N6C8 Human Leukocyte immunoglobulin-like receptor subfamily A member 3

Gene Symbol LILRA3
Gene Full Name leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member 3
Background This gene encodes a member of a family of immunoreceptors that are expressed predominantly in monocytes and B cells, and at lower levels in dendritic cells and natural killer cells. The encoded protein lacks the transmembrane region found in other members of this family. It acts as a soluble receptor for class I major histocompatibility complex (MHC) antigens. Alternatively spliced transcript variants encoding different isoforms have been found. This gene is located in a cluster of related genes on chromosome 19 and is polymorphic in human populations, with many individuals containing a deletion of this genomic region. [provided by RefSeq, Mar 2014]
Function Acts as soluble receptor for class I MHC antigens. Binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes. [UniProt]
Cellular Localization Secreted. [UniProt]
Highlight Related products:
CD85 antibodies; CD85 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM N-glycosylation is required for ligand binding. [UniProt]
Title Download Link
ARG82763 Human CD85e / LILRA3 ELISA Kit User manual download icon Download