ARG81254
Human CD95 / Fas ELISA Kit
Human CD95 / Fas ELISA Kit for ELISA and Human
Overview
| Product Description | ARG81254 Human CD95 / Fas ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD95 / Fas in serum, plasma or cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | CD95 / Fas |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 60 pg/ml |
| Sample Type | Serum, plasma or cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Alternate Names | CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen |
Application Instructions
| Assay Time | ~ 3.5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P25445 Human Tumor necrosis factor receptor superfamily member 6 |
|---|---|
| Gene Symbol | FAS |
| Gene Full Name | Fas cell surface death receptor |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt] |
| Highlight | Related products: CD95 antibodies; CD95 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Title | Download Link |
|---|---|
| ARG81254 Human CD95 / Fas ELISA Kit User's manual |
 Download
|
