ARG81572

Human CD97 ELISA Kit

Human CD97 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81572-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81572-002 Standard 2 X 10 ng/vial 4°C
ARG81572-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81572-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81572-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81572-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81572-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81572-008 25X Wash buffer 20 ml 4°C
ARG81572-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81572-010 STOP solution 10 ml (Ready to use) 4°C
ARG81572-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81572 Human CD97 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD97 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD97
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 62.5 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%
Inter-Assay CV: 6.1%
Alternate Names CD97; Leukocyte antigen CD97; CD97 antigen; CD antigen CD97; TM7LN1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 976 Human ADGRE5

Swiss-port # P48960 Human CD97 antigen

Gene Symbol ADGRE5
Gene Full Name adhesion G protein-coupled receptor E5
Background This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]
Function Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity). [UniProt]
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PTM Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. [UniProt]
Title Download Link
ARG81572 Human CD97 ELISA Kit User's manual download icon Download