ARG82709

Human CES1 ELISA Kit

Human CES1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82709-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82709-002 Standard 2 X 50 ng/vial 4°C
ARG82709-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82709-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82709-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82709-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82709-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82709-008 25X Wash buffer 20 ml 4°C
ARG82709-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82709-010 STOP solution 10 ml (Ready to use) 4°C
ARG82709-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82709 Human CES1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CES1 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CES1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.6%
Inter-Assay CV: 6.3%
Alternate Names SES1; Cocaine carboxylesterase; REH; ACAT; Serine esterase 1; HMSE; Retinyl ester hydrolase; Methylumbelliferyl-acetate deacetylase 1; hCE-1; EC 3.1.1.56; CE-1; CEH; HMSE1; TGH; PCE-1; Acyl-coenzyme A:cholesterol acyltransferase; EC 3.1.1.1; Liver carboxylesterase 1; Brain carboxylesterase hBr1; Triacylglycerol hydrolase; CES2; Egasyn; Carboxylesterase 1; Monocyte/macrophage serine esterase

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1066 Human CES1

Swiss-port # P23141 Human Liver carboxylesterase 1

Gene Symbol CES1
Gene Full Name carboxylesterase 1
Background This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Function Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. [UniProt]
Cellular Localization Endoplasmic reticulum lumen. [UniProt]
Highlight Related products:
CES antibodies; CES ELISA Kits;
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Simplify the ELISA analysis by GainData
PTM Contains sialic acid.

Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. [UniProt]
Title Download Link
ARG82709 Human CES1 ELISA Kit User manual download icon Download