ARG82710

Human CES2 ELISA Kit

Human CES2 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82710-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82710-002 Standard 2 X 10 ng/vial 4°C
ARG82710-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82710-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82710-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82710-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82710-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82710-008 25X Wash buffer 20 ml 4°C
ARG82710-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82710-010 STOP solution 10 ml (Ready to use) 4°C
ARG82710-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82710 Human CES2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CES2 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name CES2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.7%
Inter-Assay CV: 6.4%
Alternate Names hCE-2; Methylumbelliferyl-acetate deacetylase 2; EC 3.1.1.84; Carboxylesterase 2; Cocaine esterase; CE-2; iCE; EC 3.1.1.56; CES2A1; PCE-2; EC 3.1.1.1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8824 Human CES2

Swiss-port # O00748 Human Cocaine esterase

Gene Symbol CES2
Gene Full Name carboxylesterase 2
Background This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]
Function Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. [UniProt]
Cellular Localization Endoplasmic reticulum lumen. [UniProt]
Highlight Related products:
CES antibodies; CES ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Glycosylated. [UniProt]
Title Download Link
ARG82710 Human CES2 ELISA kit User manual download icon Download