ARG82420

Human CFHR5 ELISA Kit

Human CFHR5 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82420-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82420-002 Standard 2 X 50 ng/vial 4°C
ARG82420-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82420-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82420-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82420-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82420-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82420-008 25X Wash buffer 20 ml 4°C
ARG82420-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82420-010 STOP solution 10 ml (Ready to use) 4°C
ARG82420-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82420 Human CFHR5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CFHR5 in serum, plasma (EDTA, heparin), cell culture supernatants and urine.
Tested Reactivity Hu
Tested Application ELISA
Target Name CFHR5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (EDTA, heparin), cell culture supernatants and urine.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.5%
Inter-Assay CV: 5.1%
Alternate Names CFHL5; FHR-5; CFHR5D; FHR5; Complement factor H-related protein 5

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 81494 Human CFHR5

Swiss-port # Q9BXR6 Human Complement factor H-related protein 5

Gene Symbol CFHR5
Gene Full Name complement factor H-related 5
Background This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). [provided by RefSeq, Jan 2010]
Function Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. [UniProt]
Cellular Localization Secreted. [UniProt]
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