ARG81494

Human CHI3L1 ELISA Kit

Human CHI3L1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81494-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81494-002 Standard 2 X 10 ng/vial 4°C
ARG81494-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81494-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81494-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81494-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81494-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81494-008 25X Wash buffer 20 ml 4°C
ARG81494-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81494-010 STOP solution 10 ml (Ready to use) 4°C
ARG81494-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81494 Human CHI3L1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CHI3L1 in serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CHI3L1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.0%
Inter-Assay CV: 6.6%
Alternate Names YYL-40; GP-39; ASRT7; YKL40; HC-gp39; HCGP-3P; GP39; Cartilage glycoprotein 39; CGP-39; Chitinase-3-like protein 1; hCGP-39; YKL-40; 39 kDa synovial protein

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1116 Human CHI3L1

Swiss-port # P36222 Human Chitinase-3-like protein 1

Gene Symbol CHI3L1
Gene Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Background Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
Function Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. [UniProt]
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PTM Glycosylated. [UniProt]
Title Download Link
ARG81494 Human CHI3L1 ELISA Kit User's manual download icon Download