ARG81608
Human CHL1 ELISA Kit
Human CHL1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81608-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81608-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81608-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81608-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81608-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81608-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81608-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81608-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81608-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81608-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81608-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81608 Human CHL1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CHL1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CHL1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 7.0% Inter-Assay CV: 8.2% |
| Alternate Names | Close homolog of L1; L1CAM2; Neural cell adhesion molecule L1-like protein; CALL |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00533 Human Neural cell adhesion molecule L1-like protein |
|---|---|
| Gene Symbol | CHL1 |
| Gene Full Name | cell adhesion molecule L1-like |
| Background | The protein encoded by this gene is a member of the L1 gene family of neural cell adhesion molecules. It is a neural recognition molecule that may be involved in signal transduction pathways. The deletion of one copy of this gene may be responsible for mental defects in patients with 3p- syndrome. This protein may also play a role in the growth of certain cancers. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, Nov 2011] |
| Function | Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane (By similarity). [UniProt] |
| Highlight | Related products: CHL1 antibodies; CHL1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Cleavage by metalloprotease ADAM8 in the extracellular part generates 2 soluble forms (125 kDa and 165 kDa) in vitro and is inhibited by metalloprotease inhibitors. N-glycosylated. Contains N-linked oligosaccharides with a sulfated carbohydrate structure type HNK-1 (SO4-3-GlcUABeta1,3GalBeta1,4GlcNAc) (By similarity). O-glycosylated. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81608 Human CHL1 ELISA Kit User's manual |
 Download
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