ARG81478
Human COMP ELISA Kit
Human COMP ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81478-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81478-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81478-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81478-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81478-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81478-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81478-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81478-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81478-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81478-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81478-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81478 Human COMP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human COMP in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | COMP |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4% Inter-Assay CV: 5.8% |
| Alternate Names | MED; TSP5; Thrombospondin-5; COMP; THBS5; EDM1; PSACH; EPD1; Cartilage oligomeric matrix protein |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P49747 Human Cartilage oligomeric matrix protein |
|---|---|
| Gene Symbol | COMP |
| Gene Full Name | cartilage oligomeric matrix protein |
| Background | The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008] |
| Function | May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity). [UniProt] |
| Highlight | Related products: COMP antibodies; COMP ELISA Kits; COMP Duos / Panels; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG81478 Human COMP ELISA Kit User's manual |
 Download
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