ARG81478

Human COMP ELISA Kit

Human COMP ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81478-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81478-002 Standard 2 X 10 ng/vial 4°C
ARG81478-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81478-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81478-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81478-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81478-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81478-008 25X Wash buffer 20 ml 4°C
ARG81478-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81478-010 STOP solution 10 ml (Ready to use) 4°C
ARG81478-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81478 Human COMP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human COMP in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name COMP
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 5.8%
Alternate Names MED; TSP5; Thrombospondin-5; COMP; THBS5; EDM1; PSACH; EPD1; Cartilage oligomeric matrix protein

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1311 Human COMP

Swiss-port # P49747 Human Cartilage oligomeric matrix protein

Gene Symbol COMP
Gene Full Name cartilage oligomeric matrix protein
Background The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008]
Function May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity). [UniProt]
Highlight Related products:
COMP antibodies; COMP ELISA Kits; COMP Duos / Panels;
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Title Download Link
ARG81478 Human COMP ELISA Kit User's manual download icon Download