Component

Overview

Product Description	ARG81434 Human CST3 / Cystatin C ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CST3 / Cystatin C in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants.
Tested Reactivity	Hu
Tested Application	ELISA
Specificity	There is no detectable cross-reactivity with other relevant proteins.
Target Name	CST3 / Cystatin C
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm.
Sensitivity	156 pg/ml
Sample Type	Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants.
Standard Range	312 - 20000 pg/ml
Sample Volume	100 µl
Precision	Intra-Assay CV: 6.8% Inter-Assay CV: 8.1%
Alternate Names	Cystatin-C; Neuroendocrine basic polypeptide; Post-gamma-globulin; ARMD11; Cystatin-3; Gamma-trace

Application Instructions

Assay Time	~ 5 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 1471 Human CST3 Swiss-port # P01034 Human Cystatin-C
Gene Symbol	CST3
Gene Full Name	cystatin C
Background	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]
Function	As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. [UniProt]
Highlight	Related products: Cystatin antibodies; Cystatin ELISA Kits; Cystatin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData
PTM	The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. [UniProt]