ARG70038
Human CXCL1 / GRO alpha recombinant protein (Active) (His-tagged, N-ter)
Human CXCL1 / GRO alpha recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human CXCL1 / GRO alpha recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CXCL1 / GRO alpha recombinant protein |
| Species | Human |
| A.A. Sequence | ASVAT ELRCQ CLQTL QGIHP KNIQS VNVKS PGPHC AQTEV IATLK NGRKA CLNPA SPIVK KIIEK MLNSD KSN with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2. The ED50 for this effect is < 3 ng/ml. |
| Alternate Names | Growth-regulated alpha protein; SCYB1; Melanoma growth stimulatory activity; MGSA-a; GRO-alpha; GROa; NAP-3; FSP; 5-73; C-X-C motif chemokine 1; Neutrophil-activating protein 3; 6-73; 1-73; GRO1; MGSA; 4-73 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CXCL1 |
|---|---|
| Gene Full Name | chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) |
| Background | This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014] |
| Function | Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. [UniProt] |
