ARG70039
Human CXCL10 / IP10 recombinant protein (Active) (His-tagged, N-ter)
Human CXCL10 / IP10 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human CXCL10 / IP10 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CXCL10 / IP10 recombinant protein |
| Species | Human |
| A.A. Sequence | VPLSR TVRCT CISIS NQPVN PRSLE KLEII PASQF CPRVE IIATM KKKGE KRCLN PESKA IKNLL KAVSK ERSKR SP with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR3. The ED50 for this effect is < 0.15 µg/ml. |
| Alternate Names | gIP-10; INP10; 10 kDa interferon gamma-induced protein; IP-10; C-X-C motif chemokine 10; 1-73; Small-inducible cytokine B10; IFI10; Gamma-IP10; SCYB10; crg-2; C7; mob-1 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CXCL10 |
|---|---|
| Gene Full Name | chemokine (C-X-C motif) ligand 10 |
| Background | This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Sep 2014] |
| Function | Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: CXCL10 antibodies; CXCL10 ELISA Kits; CXCL10 recombinant proteins; Related news: circNDUFB2, a circular RNA (circRNA), activates anti-tumor immunity |
| PTM | CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. [UniProt] |
