ARG80121

Human CXCL12 / SDF1 alpha ELISA Kit

Human CXCL12 / SDF1 alpha ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Immune System kit; Microbiology and Infectious Disease kit

Component

Cat No Component Name Package Temp
ARG80121-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80121-002 Standard (Lyophilized) 3 X 4 ng/vial 4°C
ARG80121-003 Standard diluent buffer 20 ml 4°C
ARG80121-004 Antibody conjugate concentrate 400 µl 4°C
ARG80121-005 Antibody diluent buffer 16 ml 4°C
ARG80121-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80121-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80121-008 20X Wash buffer 50 ml 4°C
ARG80121-009 TMB substrate 12ml 4°C (Protect from light)
ARG80121-010 STOP solution 12ml 4°C
ARG80121-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80121 Human CXCL12 / SDF1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL12 / SDF1 alpha in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human beta-ECGF, GRO alpha, GRO beta, GRO gamma, MCP-1, MIP-1 alpha, MIP-1 beta, PTN, RANTES;mouse MIP-1 alpha, MIP-1 beta. The cross-reactivity tested with 50 ng/ml of Human SDF-1 beta is <10% and rat SDF-1 beta is <1%.
Target Name CXCL12 / SDF1 alpha
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 62.5 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names TPAR1; SDF1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; TLSF; PBSF; SDF-1; Intercrine reduced in hepatomas; IRH; hSDF-1; 3-72; SCYB12; hIRH; 3-67; Stromal cell-derived factor 1

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CXCL12
Gene Full Name chemokine (C-X-C motif) ligand 12
Background Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. [provide by uniprot]
Highlight Related products:
CXCL12 antibodies; CXCL12 ELISA Kits; CXCL12 recombinant proteins;
Related news:
LKB1 deficiency in T cells promotes gut tumors
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cell Biology and Cellular Response kit; Immune System kit; Microbiology and Infectious Disease kit
PTM Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
Title Download Link
ARG80121 Human CXCL12 / SDF1 alpha ELISA Kit User manual download icon Download