ARG81445

Human CXCL13 / BCA1 ELISA Kit

Human CXCL13 / BCA1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81445-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81445-002 Standard 2 X 10 ng/vial 4°C
ARG81445-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81445-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81445-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81445-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81445-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81445-008 25X Wash buffer 20 ml 4°C
ARG81445-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81445-010 STOP solution 10 ml (Ready to use) 4°C
ARG81445-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81445 Human CXCL13 / BCA1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL13 / BCA1 in serum, plasma (heparin, EDTA), saliva and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CXCL13 / BCA1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA), saliva and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.8%
Inter-Assay CV: 7.7%
Alternate Names BCA-1; Angie; C-X-C motif chemokine 13; CXC chemokine BLC; ANGIE2; B lymphocyte chemoattractant; B cell-attracting chemokine 1; BLR1L; Small-inducible cytokine B13; SCYB13; ANGIE; BLC; BCA1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10563 Human CXCL13

Swiss-port # O43927 Human C-X-C motif chemokine 13

Gene Symbol CXCL13
Gene Full Name chemokine (C-X-C motif) ligand 13
Background B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq, Oct 2014]
Function Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. [UniProt]
Highlight Related products:
CXCL antibodies; CXCL ELISA Kits; CXCL Duos / Panels; CXCL recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Title Download Link
ARG81445 Human CXCL13 / BCA1 ELISA Kit User's manual download icon Download