ARG81547

Human CXCL14 ELISA Kit

Human CXCL14 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81547-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81547-002 Standard 2 X 10 ng/vial 4°C
ARG81547-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81547-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81547-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81547-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81547-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81547-008 25X Wash buffer 20 ml 4°C
ARG81547-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81547-010 STOP solution 10 ml (Ready to use) 4°C
ARG81547-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81547 Human CXCL14 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CXCL14 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Predict Reactivity Bov, Hm, Hrs, Mk
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CXCL14
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%
Inter-Assay CV: 7.3%
Alternate Names MIP-2G; KS1; BMAC; Small-inducible cytokine B14; KEC; C-X-C motif chemokine 14; MIP-2g; Chemokine BRAK; MIP2G; BRAK; NJAC; SCYB14

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9547 Human CXCL14

Swiss-port # O95715 Human C-X-C motif chemokine 14

Gene Symbol CXCL14
Gene Full Name chemokine (C-X-C motif) ligand 14
Background This antimicrobial gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. [provided by RefSeq, Sep 2014]
Function Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. [UniProt]
Highlight Related products:
CXCL antibodies; CXCL ELISA Kits; CXCL Duos / Panels; CXCL recombinant proteins;
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Simplify the ELISA analysis by GainData
PTM Ubiquitinated, followed by degradation by the proteasome. [UniProt]
Title Download Link
ARG81547 Human CXCL14 ELISA Kit User's manual download icon Download